Detection of Anti-KHV DNA Vaccine Transmission toward Bacteria in Common Carp Cultivation Media

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KHV is an infectious disease that attacks common carp in various stadia and causing 80-100% of death. One of ways to prevent KHV infection is by using DNA vaccine. DNA vaccine is a genetically modified product that was feared to be transmitted to bacteria and affects genetic diversity. This research was performed to detect of anti-KHV DNA vaccine transmission into bacteria of common carp rearing water. Vaccination was conducted by dipping and oral vaccination methods. Bacteria from dipping vaccination and aquaria were isolated on 6 hours, 4th day, and 7th day post vaccination while bacteria from pond were isolated on 6 hours, 4th day and 7th day after fish were reared in pond. Bacteria as many as 50 μL were spread in triptic soy agar containing ampicilline (50 μg/μL). Bacteria that grow in the TSA contain ampisilin were 103 bacteria and the amount of bacteria that identified to species level by using API kit were 11 bacteria; 2 colonies were identified as Aeromonas hydrophila, 4 colonies were identified as Bacillus cereus, 2 colonies were identified as Bacillus subtilis, 2 colonies were identified as Staphylococcus auricularis dan 1 colony was identified as Enterobacter sakazakii. The existence of DNA vaccine in the bacteria was tested by using cracking and PCR methods. The results of cracking indicated the absence of bacteria containing anti-KHV DNA vaccine. Analysis of bacteria by PCR method showed that the bacteria could not uptake anti-KHV DNA vaccine from the environment, so the conclusion was anti-KHV DNA vaccine did not transmitted to bacteria in common carp rearing water.
Keywords: KHV, DNA vaccine, transmission, bacteria in the water, common carp.

Deteksi Transmisi Vaksin DNA Anti-KHV pada Bakteri di Media Budidaya Ikan Mas

KHV merupakan penyakit menular yang menyerang ikan mas dalam berbagai stadia, dapat mengakibatkan kematian 80-100%. Salah satu cara penanggulangan serangan KHV adalah dengan penggunaan vaksin DNA. Vaksin DNA merupakan produk rekayasa genetika yang dikhawatirkan dapat melakukan transmisi pada bakteri di perairan dan mempengaruhi keragaman hayati. Penelitian ini bertujuan untuk mendeteksi transmisi vaksin DNA anti-KHV ke bakteri di air pemeliharaan ikan mas. Vaksinasi dilakukan menggunakan metode perendaman, dan pemberian melalui pakan. Bakteri dari air vaksinasi melalui perendaman dan akuarium pemeliharaan ikan mas diisolasi pada 6 jam, hari ke-4, dan hari ke-7 pascavaksinasi, sedangkan bakteri dari kolam diisolasi setelah 6 jam, hari ke-4 dan hari ke-7 ikan dipelihara di kolam. Bakteri tersebut disebar sebanyak 50 μL pada media kultur mengandung ampisilin (50 μg/μL). Bakteri yang tumbuh pada media TSA yang mengandung ampisilin sebanyak 103 bakteri dan yang teridentifikasi hingga tingkat spesies dengan menggunakank kit API ada 11 bakteri, yakni 2 Aeromonas hydrophila, 4 Bacillus cereus, 2 B. subtilis, 2 Staphylococcus auricularis dan 1 Enterobacter sakazakii. Keberadaan vaksin DNA dalam bakteri diuji menggunakan metode cracking, dan PCR. Hasil cracking menunjukkan tidak adanya bakteri yang mengandung vaksin DNA anti-KHV. Analisis bakteri dengan metode PCR menunjukkan bahwa bakteri tidak dapat meng-uptake atau mengambil vaksin DNA anti-KHV dari lingkungan, sehingga dapat disimpulkan bahwa vaksin DNA anti-KHV tidak ditransmisikan ke bakteri media pemeliharaan ikan mas.

Kata kunci: KHV, Vaksin DNA, transmisi, bakteri dalam air, ikan mas.
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